SYBR Green qPCR primers for detecting mouse Ap4s1 (adaptor-related protein complex AP-4, sigma 1, also called Sigma4-adaptin) gene expression by real-time PCR.
Mouse Ap4s1 qPCR Primer Set – adaptor-related protein complex AP-4, sigma 1
About this gene: Ap4s1 encodes a sigma subunit of the adaptor-related protein complex 4 which mediates intracellular membrane trafficking along the endocytic and secretory transport pathways. This complex contains four subunits, beta, epsilon, mu, and sigma, and belongs to a family of five adapter protein complexes, including three clathrin-associated complexes and two non clathrin-associated complexes. In humans, loss-of-function mutations in Ap4s1 have been linked to specific adapter complex 4 deficiency disorders including hereditary spastic paraplegia.
Gene details: NCBI Gene ID: 11782; UniProt: Q9WVL1; Chromosome location: 12 B3|12 22.11 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.