SYBR Green qPCR primers for detecting mouse Lig4 (ligase IV, DNA, ATP-dependent) gene expression by real-time PCR.
Mouse Lig4 qPCR Primer Set – ligase IV, DNA, ATP-dependent
Also known as: 5830471N16Rik, tiny
About this gene: Lig4 enables ATP binding activity and DNA ligase activity. It acts upstream of or within multiple processes, including hemopoiesis; response to gamma radiation; and somatic diversification of immune receptors via germline recombination within a single locus.
Gene details: NCBI Gene ID: 319583; UniProt: Q8BTF7; Chromosome location: 8 A1.1|8 4.52 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.