SYBR Green qPCR primers for detecting mouse Lcmt2 (leucine carboxyl methyltransferase 2, also called Phe) gene expression by real-time PCR.
Mouse Lcmt2 qPCR Primer Set – leucine carboxyl methyltransferase 2
Also known as: D330024M17, Tyw4
About this gene: Lcmt2 is predicted to enable protein C-terminal carboxyl O-methyltransferase activity and tRNA methyltransferase activity. It is predicted to be involved in tRNA methylation and wybutosine biosynthetic process. It is predicted to act upstream of or within C-terminal protein methylation.
Gene details: NCBI Gene ID: 329504; UniProt: Q8BYR1; Chromosome location: 2 E5|2 60.37 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.