SYBR Green qPCR primers for detecting mouse Large1 (LARGE xylosyl- and glucuronyltransferase 1) gene expression by real-time PCR.
Mouse Large1 qPCR Primer Set – LARGE xylosyl- and glucuronyltransferase 1
Also known as: BPFD#36, Gyltl1a, Large, Mbp-1, Mbp1, enr, fg, froggy, myd
About this gene: Large1 enables glucuronosyltransferase activity and xylosyltransferase activity. It is involved in muscle cell cellular homeostasis; protein O-linked glycosylation via mannose; and skeletal muscle organ development. It acts upstream of or within multiple processes, including nervous system development; retina development in camera-type eye; and striated muscle cell differentiation.
Gene details: NCBI Gene ID: 16795; UniProt: Q9Z1M7; Chromosome location: 8 B3.3|8 35.08 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.