SYBR Green qPCR primers for detecting mouse Itch (itchy, E3 ubiquitin protein ligase) gene expression by real-time PCR.
Mouse Itch qPCR Primer Set – itchy, E3 ubiquitin protein ligase
Also known as: 6720481N21Rik, 8030492O04Rik, A130065M08, AIP4, C230047C07Rik
About this gene: Itch enables ligase activity. It is involved in multiple processes, including negative regulation of defense response to virus; negative regulation of intracellular signal transduction; and protein K48-linked ubiquitination. It acts upstream of or within multiple processes, including negative regulation of alpha-beta T cell proliferation; positive regulation of T cell anergy; and positive regulation of protein catabolic process.
Gene details: NCBI Gene ID: 16396; UniProt: Q8C863; Chromosome location: 2 H1|2 76.94 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.