SYBR Green qPCR primers for detecting mouse Hspa5 (heat shock protein family A (Hsp70) member 5, also called BiP) gene expression by real-time PCR.
Mouse Hspa5 qPCR Primer Set – heat shock protein family A (Hsp70) member 5
Also known as: Bip, D2Wsu141e, D2Wsu17e, Grp78, Hsce70, SEZ-7, Sez7, baffled, mBiP
About this gene: Hspa5 enables misfolded protein binding activity; protein folding chaperone; and ribosome binding activity. It is involved in IRE1-mediated unfolded protein response; cerebellum development; and positive regulation of protein metabolic process. It acts upstream of or within ER overload response; cellular response to interleukin-4; and negative regulation of transforming growth factor beta receptor signaling pathway.
Gene details: NCBI Gene ID: 14828; UniProt: P20029; Chromosome location: 2 B|2 22.94 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.