SYBR Green qPCR primers for detecting mouse Hk2 (hexokinase 2) gene expression by real-time PCR.
Mouse Hk2 qPCR Primer Set – hexokinase 2
Also known as: HKII
About this gene: Hk2 enables hexokinase activity. It is involved in negative regulation of mitochondrial membrane permeability and negative regulation of reactive oxygen species metabolic process. It acts upstream of or within multiple processes, including carbohydrate phosphorylation; cellular response to leukemia inhibitory factor; and regulation of D-glucose import across plasma membrane.
Gene details: NCBI Gene ID: 15277; UniProt: O08528; Chromosome location: 6 C3|6 35.94 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.