SYBR Green qPCR primers for detecting mouse Hhat (hedgehog acyltransferase) gene expression by real-time PCR.
Mouse Hhat qPCR Primer Set – hedgehog acyltransferase
Also known as: 2810432O22Rik, AP-2CRE, Skn, Tg(TFAP2A-cre)1Will
About this gene: Hhat enables protein-cysteine S-palmitoyltransferase activity. It is involved in positive regulation of smoothened signaling pathway and protein maturation. It acts upstream of or within protein palmitoylation and smoothened signaling pathway.
Gene details: NCBI Gene ID: 226861; UniProt: Q8BMT9; Chromosome location: 1 H6|1 97.55 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.