SYBR Green qPCR primers for detecting mouse Hgf (hepatocyte growth factor, also called SF) gene expression by real-time PCR.
Mouse Hgf qPCR Primer Set – hepatocyte growth factor
Also known as: C230052L06Rik, HGF/SF, NK1, NK2, SF, SF/HGF
About this gene: Hgf encodes a protein that binds to the hepatocyte growth factor receptor to regulate cell growth, cell motility and morphogenesis in numerous cell and tissue types. The encoded preproprotein is proteolytically processed to generate multiple protein products, including the hepatocyte growth factor alpha and beta chains, which heterodimerize to form the mature active protein. Although this protein belongs to the peptidase S1 family of serine proteases, it lacks peptidase activity.
Gene details: NCBI Gene ID: 15234; UniProt: Q08048; Chromosome location: 5 A3|5 7.07 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.