SYBR Green qPCR primers for detecting mouse Hdac3 (histone deacetylase 3, also called HD3) gene expression by real-time PCR.
Mouse Hdac3 qPCR Primer Set – histone deacetylase 3
About this gene: Hdac3 enables chromatin DNA binding activity; histone deacetylase activity; and histone decrotonylase activity. It is involved in multiple processes, including cornified envelope assembly; establishment of skin barrier; and regulation of gene expression. It acts upstream of or within multiple processes, including negative regulation of cardiac muscle cell differentiation; negative regulation of transcription by RNA polymerase II; and regulation of mitotic cell cycle.
Gene details: NCBI Gene ID: 15183; UniProt: O88895; Chromosome location: 18 19.81 cM|18 B3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.