SYBR Green qPCR primers for detecting mouse Hars1 (histidyl-tRNA synthetase 1, also called HisRS) gene expression by real-time PCR.
Mouse Hars1 qPCR Primer Set – histidyl-tRNA synthetase 1
Also known as: Hars, MMHRS
About this gene: Hars1 is predicted to enable multiple functions, including ATP binding activity; histidine-tRNA ligase activity; and protein homodimerization activity. It is predicted to be involved in histidyl-tRNA aminoacylation. Predicted to be active in cytosol.
Gene details: NCBI Gene ID: 15115; UniProt: Q61035; Chromosome location: 18 B2|18 19.46 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.