SYBR Green qPCR primers for detecting mouse Gtf3a (general transcription factor III A, also called TFIIIA) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Gtf3a qPCR Primer Set – general transcription factor III A
Also known as: 2010015D03Rik, 2610111I01Rik, 5330403M05Rik
About this gene: The product of Gtf3a is a zinc finger protein with nine Cis[2]-His[2] zinc finger domains. The Gtf3a gene product acts as an RNA polymerase III transcription factor to induce transcription of the 5S rRNA genes. The protein binds to a 50 bp internal promoter in the 5S genes called the internal control region, and nucleates formation of a stable preinitiation complex.
Gene details: NCBI Gene ID: 66596; UniProt: Q8VHT7; Chromosome location: 5 G3|5 86.57 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.