SYBR Green qPCR primers for detecting mouse Alox5 (arachidonate 5-lipoxygenase) gene expression by real-time PCR.
Mouse Alox5 qPCR Primer Set – arachidonate 5-lipoxygenase
Also known as: 5-LO, 5-LOX, 5LO, 5LX, F730011J02
About this gene: Alox5 enables arachidonate 5-lipoxygenase activity. It is involved in multiple processes, including leukotriene A4 biosynthetic process; negative regulation of angiogenesis; and regulation of cellular response to stress. It acts upstream of or within leukotriene biosynthetic process and leukotriene production involved in inflammatory response.
Gene details: NCBI Gene ID: 11689; UniProt: P48999; Chromosome location: 6 E3|6 53.79 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.