SYBR Green qPCR primers for detecting mouse Alox12 (arachidonate 12-lipoxygenase, also called P-12LO) gene expression by real-time PCR.
Mouse Alox12 qPCR Primer Set – arachidonate 12-lipoxygenase
Also known as: 9930022G08Rik, Alox12p, P-12LO
About this gene: Alox12 enables arachidonate 12-lipoxygenase activity and linoleate 13S-lipoxygenase activity. It is involved in establishment of skin barrier; fatty acid metabolic process; and negative regulation of platelet aggregation. Is active in cytosol.
Gene details: NCBI Gene ID: 11684; UniProt: P39655; Chromosome location: 11 B3|11 42.99 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.