SYBR Green qPCR primers for detecting mouse Got2 (glutamatic-oxaloacetic transaminase 2, mitochondrial, also called mAspAT) gene expression by real-time PCR.
Mouse Got2 qPCR Primer Set – glutamatic-oxaloacetic transaminase 2, mitochondrial
Also known as: FABP-pm, Got-2, Kyat4, mAspAT
About this gene: Got2 enables L-aspartate:2-oxoglutarate transaminase activity. It is involved in L-asparagine biosynthetic process; L-glutamine catabolic process; and malate-aspartate shuttle. It acts upstream of or within L-aspartate biosynthetic process; L-glutamate catabolic process; and oxaloacetate metabolic process.
Gene details: NCBI Gene ID: 14719; UniProt: P05202; Chromosome location: 8 D1|8 47.79 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.