SYBR Green qPCR primers for detecting mouse Aldh6a1 (aldehyde dehydrogenase family 6, subfamily A1, also called MMSDH) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Aldh6a1 qPCR Primer Set – aldehyde dehydrogenase family 6, subfamily A1
Also known as: 1110038I05Rik, Mmsdh
About this gene: Aldh6a1 belongs to the aldehyde dehydrogenase protein family. The encoded enzyme is a mitochondrial methylmalonate semialdehyde dehydrogenase that is involved in the valine and pyrimidine catabolic pathways. This enzyme catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Mutations in the human gene result in Methylmalonate Semialdehyde Dehydrogenase Deficiency, characterized by elevated beta-alanine, 3-hydroxypropionic acid.
Gene details: NCBI Gene ID: 104776; UniProt: Q9EQ20; Chromosome location: 12 D1|12 39.21 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.