SYBR Green qPCR primers for detecting mouse Akap3 (A kinase anchor protein 3, also called PRKA3) gene expression by real-time PCR.
Mouse Akap3 qPCR Primer Set – A kinase anchor protein 3
Also known as: Prka3
About this gene: Akap3 enables protein kinase A binding activity. It is involved in establishment of protein localization and flagellated sperm motility. It acts upstream of or within blastocyst hatching; cell surface receptor protein serine/threonine kinase signaling pathway; and intracellular protein localization.
Gene details: NCBI Gene ID: 11642; UniProt: O88987; Chromosome location: 6 F3|6 61.92 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.