SYBR Green qPCR primers for detecting mouse Fhit (fragile histidine triad gene, also called AP3Aase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Fhit qPCR Primer Set – fragile histidine triad gene
Also known as: Fra14A2
About this gene: Fhit belongs to the HIT family of proteins that are characterized by the presence of a histidine triad sequence. The Fhit protein is a diadenosine triphosphate hydrolase enzyme that cleaves the P-P-bis triphosphate to yield AMP and ADP. This locus is very fragile and has been found to be altered in different types of cancers. Mice lacking the Fhit protein display increased susceptibility to spontaneous and induced tumors.
Gene details: NCBI Gene ID: 14198; UniProt: O89106; Chromosome location: 14 A1|14 5.61 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.