SYBR Green qPCR primers for detecting mouse F7 (coagulation factor VII) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse F7 qPCR Primer Set – coagulation factor VII
Also known as: Cf7, FVII
About this gene: F7 encodes a vitamin K-dependent serine protease that plays a critical role in the extrinsic pathway of blood coagulation. Upon contact with tissue factor III, the F7 protein forms an activated complex termed TF-FVIIa that initiates the coagulation cascade involving other coagulation factors, ultimately resulting in a fibrin clot. Complete lack of the F7 protein in mice leads to in perinatal lethality due to bleeding from normal blood vessels.
Gene details: NCBI Gene ID: 14068; UniProt: P70375; Chromosome location: 8 A1.1|8 5.73 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.