SYBR Green qPCR primers for detecting mouse F3 (coagulation factor III, tissue factor, also called TF) gene expression by real-time PCR.
Mouse F3 qPCR Primer Set – coagulation factor III, tissue factor
Also known as: CD142, Cf-3, Cf3, TF
About this gene: F3 encodes a membrane-bound glycoprotein that forms the primary physiological initiator of the blood coagulation process following vascular damage. A severe deficiency of the F3 protein in mice leads to impaired uterine homeostasis, shorter life spans due to spontaneous fatal hemorrhages and cardiac fibrosis.
Gene details: NCBI Gene ID: 14066; UniProt: P20352; Chromosome location: 3 G1|3 52.94 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.