SYBR Green qPCR primers for detecting mouse Ehhadh (enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase, also called PBFE) gene expression by real-time PCR.
Mouse Ehhadh qPCR Primer Set – enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
Also known as: 1300002P22Rik, HD, L-PBE, LBFP, LBP, MFE1, MFP, MFP-1, MFP1, PBFE
About this gene: Enables-3-hydroxyacyl-CoA dehydrogenase activity and 3-hydroxyacyl-CoA dehydratase activity. It is involved in fatty acid beta-oxidation using acyl-CoA oxidase and fatty acid biosynthetic process. It acts upstream of or within fatty acid beta-oxidation.
Gene details: NCBI Gene ID: 74147; UniProt: Q9DBM2; Chromosome location: 16 B1|16 12.86 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.