SYBR Green qPCR primers for detecting mouse Dmd (dystrophin, muscular dystrophy) gene expression by real-time PCR.
Mouse Dmd qPCR Primer Set – dystrophin, muscular dystrophy
Also known as: DXSmh7, DXSmh9, Dp427, Dp71, dys, mdx, pke
About this gene: Dmd encodes a large, rod-like cytoskeletal protein which is found at the inner surface of muscle fibers in skeletal and cardiac muscles. The Dmd protein is required for proper development and organization of myofibers as contractile units in striated muscles. Mutations in the human gene cause Duchenne and Becker Muscular Dystrophies and a form of heart disease called DMD-associated dilated cardiomyopathy.
Gene details: NCBI Gene ID: 13405; UniProt: P11531; Chromosome location: X C1|X 38.38 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.