SYBR Green qPCR primers for detecting mouse Dgkg (diacylglycerol kinase, gamma, also called DGK-gamma) gene expression by real-time PCR.
Mouse Dgkg qPCR Primer Set – diacylglycerol kinase, gamma
Also known as: 2900055E17Rik, 90kDa, Dagk3, E430001K23Rik, mKIAA4131
About this gene: Dgkg enables ATP-dependent diacylglycerol kinase activity. It is involved in multiple processes, including glycerolipid metabolic process; lipid phosphorylation; and negative regulation of phospholipase C/protein kinase C signal transduction. Predicted to be active in plasma membrane.
Gene details: NCBI Gene ID: 110197; UniProt: Q91WG7; Chromosome location: 16 B1|16 13.37 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.