SYBR Green qPCR primers for detecting mouse Det1 (DET1 partner of COP1 E3 ubiquitin ligase) gene expression by real-time PCR.
Mouse Det1 qPCR Primer Set – DET1 partner of COP1 E3 ubiquitin ligase
Also known as: 2610034H20Rik
About this gene: Det1 is predicted to enable ubiquitin protein ligase binding activity and ubiquitin-like ligase-substrate adaptor activity. It is predicted to be involved in positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination; and protein-containing complex assembly. Predicted to be active in nucleus.
Gene details: NCBI Gene ID: 76375; UniProt: Q9D0A0; Chromosome location: 7 44.81 cM|7 D2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.