SYBR Green qPCR primers for detecting mouse Cyp8b1 (cytochrome P450, family 8, subfamily b, polypeptide 1, also called CYPVIIIB1) gene expression by real-time PCR.
Mouse Cyp8b1 qPCR Primer Set – cytochrome P450, family 8, subfamily b, polypeptide 1
About this gene: Cyp8b1 is predicted to enable sterol 12-alpha-hydroxylase activity. It is involved in bile acid biosynthetic process and positive regulation of intestinal cholesterol absorption.
Gene details: NCBI Gene ID: 13124; UniProt: O88962; Chromosome location: 9 72.73 cM|9 F4
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.