SYBR Green qPCR primers for detecting mouse Cyld (CYLD lysine 63 deubiquitinase) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Mouse Cyld qPCR Primer Set – CYLD lysine 63 deubiquitinase
Also known as: 2010013M14Rik, 2900009M21Rik, C130039D01Rik, CDMT, CYLD1, EAC, mKIAA0849
About this gene: Cyld encodes a protein that belongs to the ubiquitin C-terminal hydrolase subfamily of the deubiquitinating enzyme family. Members of this family catalyze the removal of ubiquitin from a substrate or another ubiquitin molecule and thereby play important roles in regulating signaling pathways, recycling ubiquitin and regulating protein stability. The Cyld protein removes ubiquitin from K-63-linked ubiquitin chains from proteins involved in NF-kappaB signaling and thus acts as a negative regulator of this pathway.
Gene details: NCBI Gene ID: 74256; UniProt: Q80TQ2; Chromosome location: 8 C3|8 43.51 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Mouse PCR Primer Sets.