SYBR Green qPCR primers for detecting mouse Crybb1 (crystallin, beta B1) gene expression by real-time PCR.
Mouse Crybb1 qPCR Primer Set – crystallin, beta B1
Also known as: 3110006K12Rik, BB1CRY
About this gene: Crybb1 belongs to the crystallin family of proteins that contribute to the transparency and refractive properties of the ocular lens. The Crybb1 protein associates with other beta crystallin proteins to form dimers, tetramers and other higher-order complexes. Crybb1 is located adjacent to a related crystallin gene on chromosome 5.
Gene details: NCBI Gene ID: 12960; UniProt: Q9WVJ5; Chromosome location: 5 F|5 54.63 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.