SYBR Green qPCR primers for detecting mouse Crot (carnitine O-octanoyltransferase, also called COT) gene expression by real-time PCR.
Mouse Crot qPCR Primer Set – carnitine O-octanoyltransferase
Also known as: 1200003H03Rik
About this gene: Crot enables carnitine O-octanoyltransferase activity. It is involved in carnitine metabolic process; coenzyme A metabolic process; and fatty acid metabolic process. It acts upstream of or within fatty acid transport.
Gene details: NCBI Gene ID: 74114; UniProt: Q9DC50; Chromosome location: 5 A1|5 3.5 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.