SYBR Green qPCR primers for detecting mouse Cpe (carboxypeptidase E, also called CPH) gene expression by real-time PCR.
Mouse Cpe qPCR Primer Set – carboxypeptidase E
Also known as: CPH, Cph-1, Cph1, NF-alpha1, fat
About this gene: Cpe encodes carboxypeptidase E, a prohormone-processing exopeptidase found in secretory granules of endocrine and neuroendocrine cells. The encoded preproprotein undergoes proteolytic processing to generate a mature, functional enzyme that cleaves the C-terminal basic residues of protein substrates. A missense mutation in Cpe is responsible for the obesity phenotype in a mouse model known as the "fat mouse." Mice lacking the functional product of Cpe exhibit impaired processing of multiple peptide hormones such as proinsulin, prodynorphin.
Gene details: NCBI Gene ID: 12876; UniProt: Q00493; Chromosome location: 8 B3.1|8 32.3 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.