SYBR Green qPCR primers for detecting mouse Col27a1 (collagen, type XXVII, alpha 1, also called XXVII) gene expression by real-time PCR.
Mouse Col27a1 qPCR Primer Set – collagen, type XXVII, alpha 1
Also known as: 5730512J02Rik
About this gene: Col27a1 encodes a alpha-1 subunit of type XXVII collagen, one of the low abundance fibril-forming collagens found in cartilage. The Col27a1 protein forms a homotrimeric triple helical procollagen that undergoes proteolytic processing during fibril formation. Transgenic mice lacking a portion of the collagenous domain in the Col27a1 protein exhibit skeletal abnormalities, chondrodysplasia and die at birth because of a lung defect.
Gene details: NCBI Gene ID: 373864; UniProt: Q5QNQ9; Chromosome location: 4 B3|4 33.96 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.