SYBR Green qPCR primers for detecting mouse Cntf (ciliary neurotrophic factor) gene expression by real-time PCR.
Mouse Cntf qPCR Primer Set – ciliary neurotrophic factor
About this gene: The Cntf protein is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes, and it may be involved in reducing tissue destruction during inflammatory attacks. Read-through transcription of Zfp91 and Cntf has been observed in both human and mouse.
Gene details: NCBI Gene ID: 12803; UniProt: P51642; Chromosome location: 19 A|19 8.73 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.