SYBR Green qPCR primers for detecting mouse Clgn (calmegin, also called A2/6) gene expression by real-time PCR.
Mouse Clgn qPCR Primer Set – calmegin
Also known as: 4930459O04Rik, A2/6, Cln
About this gene: Clgn belongs to the calreticulin family, which includes calreticulin, calnexin, and calmegin, and encodes a calcium-binding molecular chaperone specifically expressed in pachytene stage male germ cells. Clgn is central to spermatogenesis and male infertility.
Gene details: NCBI Gene ID: 12745; UniProt: P52194; Chromosome location: 8 C2|8 39.97 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.