SYBR Green qPCR primers for detecting mouse Cldn12 (claudin 12) gene expression by real-time PCR.
Mouse Cldn12 qPCR Primer Set – claudin 12
About this gene: Cldn12 belongs to the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions.
Gene details: NCBI Gene ID: 64945; UniProt: Q9ET43; Chromosome location: 5 A1|5 2.98 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.