SYBR Green qPCR primers for detecting mouse Adam10 (a disintegrin and metallopeptidase domain 10) gene expression by real-time PCR.
Mouse Adam10 qPCR Primer Set – a disintegrin and metallopeptidase domain 10
Also known as: 1700031C13Rik, MADM, kuz, kuzbanian
About this gene: Adam10 belongs to a disintegrin and metalloprotease family of endoproteases that play important roles in various biological processes including cell signaling, adhesion and migration. The encoded preproprotein undergoes proteolytic processing to generate a mature enzyme that is involved in the proteolytic release of membrane-bound proteins in a process called ectodomain shedding. Mice lacking the Adam10 protein die in utero with multiple defects of the developing central nervous system, somites, and cardiovascular system.
Gene details: NCBI Gene ID: 11487; UniProt: O35598; Chromosome location: 9 D|9 39.53 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.