SYBR Green qPCR primers for detecting mouse Chrm4 (cholinergic receptor, muscarinic 4) gene expression by real-time PCR.
Mouse Chrm4 qPCR Primer Set – cholinergic receptor, muscarinic 4
Also known as: AChR M4, Chrm-4, M4, mm4 mAChR
About this gene: Chrm4 enables G protein-coupled acetylcholine receptor activity. It acts upstream of or within adenylate cyclase-inhibiting G protein-coupled acetylcholine receptor signaling pathway.
Gene details: NCBI Gene ID: 12672; UniProt: P32211; Chromosome location: 2 E1|2 50.63 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.