SYBR Green qPCR primers for detecting mouse Chpf (chondroitin polymerizing factor) gene expression by real-time PCR.
Mouse Chpf qPCR Primer Set – chondroitin polymerizing factor
Also known as: 1700028N03Rik, D1Bwg1363e
About this gene: Chpf enables N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase activity and glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase activity. It acts upstream of or within chondroitin sulfate proteoglycan biosynthetic process. Is active in Golgi apparatus.
Gene details: NCBI Gene ID: 74241; UniProt: Q6IQX7; Chromosome location: 1 C4|1 39.14 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.