SYBR Green qPCR primers for detecting mouse Actn3 (actinin alpha 3) gene expression by real-time PCR.
Mouse Actn3 qPCR Primer Set – actinin alpha 3
About this gene: Actn3 belongs to the alpha-actin binding protein gene family. The Actn3 protein is primarily expressed in skeletal muscle and acts as a structural component of sarcomeric Z line. The Actn3 protein is involved in crosslinking actin containing thin filaments.
Gene details: NCBI Gene ID: 11474; UniProt: O88990; Chromosome location: 19 A|19 4.12 cM
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.