SYBR Green qPCR primers for detecting human UPF2 (UPF2 regulator of nonsense mediated mRNA decay, also called hUpf2) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human UPF2 qPCR Primer Set – UPF2 regulator of nonsense mediated mRNA decay
Also known as: HUPF2, RENT2, smg-3
About this gene: UPF2 encodes a protein that is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay. When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. The UPF2 gene product interacts with translation release factors and the proteins that are functional homologs of yeast Upf1p and Upf3p.
Gene details: NCBI Gene ID: 26019; HGNC ID: HGNC:17854; UniProt: Q9HAU5; RefSeq (MANE Select): NM_015542.4; Chromosome location: 10p14
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.