SYBR Green qPCR primers for detecting human TYMS (thymidylate synthetase, also called TS) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human TYMS qPCR Primer Set – thymidylate synthetase
Also known as: DKCD, HST422, TMS, TS
About this gene: Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate as a cofactor. This function maintains the dTMP pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents.
Gene details: NCBI Gene ID: 7298; HGNC ID: HGNC:12441; UniProt: P04818; RefSeq (MANE Select): NM_001071.4; Chromosome location: 18p11.32
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.