SYBR Green qPCR primers for detecting human TOP2A (DNA topoisomerase II alpha) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human TOP2A qPCR Primer Set – DNA topoisomerase II alpha
Also known as: TOP2, TOP2alpha, TOPIIA, TP2A
About this gene: TOP2A encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. The TOP2A gene product catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another.
Gene details: NCBI Gene ID: 7153; HGNC ID: HGNC:11989; UniProt: P11388; RefSeq (MANE Select): NM_001067.4; Chromosome location: 17q21.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.