SYBR Green qPCR primers for detecting human BUB3 (BUB3 mitotic checkpoint protein) gene expression by real-time PCR.
Human BUB3 qPCR Primer Set – BUB3 mitotic checkpoint protein
Also known as: BUB3L, hBUB3
About this gene: BUB3 encodes a protein involved in spindle checkpoint function. The BUB3 protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Gene details: NCBI Gene ID: 9184; HGNC ID: HGNC:1151; UniProt: O43684; RefSeq (MANE Select): NM_004725.4; Chromosome location: 10q26.13
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.