SYBR Green qPCR primers for detecting human BTC (betacellulin) gene expression by real-time PCR.
Human BTC qPCR Primer Set – betacellulin
About this gene: BTC belongs to the epidermal growth factor family of proteins. A secreted form and a membrane-anchored form of this protein bind to multiple different EGF receptors. The BTC protein promotes pancreatic cell proliferation and insulin secretion, as well as retinal vascular permeability.
Gene details: NCBI Gene ID: 685; HGNC ID: HGNC:1121; UniProt: P35070; RefSeq (MANE Select): NM_001729.4; Chromosome location: 4q13.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.