SYBR Green qPCR primers for detecting human SUCLA2 (succinate-CoA ligase ADP-forming subunit beta, also called A-SCS) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human SUCLA2 qPCR Primer Set – succinate-CoA ligase ADP-forming subunit beta
Also known as: A-BETA, A-SCS, LINC00444, MTDPS5, SCS-betaA
About this gene: Succinyl-CoA synthetase is a mitochondrial matrix enzyme that acts as a heterodimer, being composed of an invariant alpha subunit and a substrate-specific beta subunit. The SUCLA2 protein is an ATP-specific SCS beta subunit that dimerizes with the SCS alpha subunit to form SCS-A, an essential component of the tricarboxylic acid cycle. SCS-A hydrolyzes ATP to convert succinate to succinyl-CoA. Defects in SUCLA2 are a cause of myopathic mitochondrial DNA depletion syndrome.
Gene details: NCBI Gene ID: 8803; HGNC ID: HGNC:11448; UniProt: Q9P2R7; RefSeq (MANE Select): NM_003850.3; Chromosome location: 13q14.2
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.