SYBR Green qPCR primers for detecting human SPINK5 (serine peptidase inhibitor Kazal type 5, also called LEKTI) gene expression by real-time PCR.
Human SPINK5 qPCR Primer Set – serine peptidase inhibitor Kazal type 5
Also known as: LEKTI, LETKI, NETS, NS, VAKTI
About this gene: SPINK5 encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia.
Gene details: NCBI Gene ID: 11005; HGNC ID: HGNC:15464; UniProt: Q9NQ38; RefSeq (MANE Select): NM_006846.4; Chromosome location: 5q32
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.