SYBR Green qPCR primers for detecting human SLC29A1 (solute carrier family 29 member 1 (Augustine blood group), also called hENT1) gene expression by real-time PCR.
Human SLC29A1 qPCR Primer Set – solute carrier family 29 member 1 (Augustine blood group)
Also known as: AUG, ENT1, hENT1
About this gene: SLC29A1 belongs to the equilibrative nucleoside transporter family. The gene encodes a transmembrane glycoprotein that localizes to the plasma and mitochondrial membranes and mediates the cellular uptake of nucleosides from the surrounding medium. The protein is categorized as an equilibrative transporter that is sensitive to inhibition by nitrobenzylthioinosine.
Gene details: NCBI Gene ID: 2030; HGNC ID: HGNC:11003; UniProt: Q99808; RefSeq (MANE Select): NM_001372327.1; Chromosome location: 6p21.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.