SYBR Green qPCR primers for detecting human BMP15 (bone morphogenetic protein 15, also called GDF-9B) gene expression by real-time PCR.
Human BMP15 qPCR Primer Set – bone morphogenetic protein 15
Also known as: GDF9B, ODG2, POF4
About this gene: BMP15 encodes a secreted ligand of the TGF-beta superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate subunits of a disulfide-linked homodimer, or alternatively, a heterodimer, with the related protein, growth differentiation factor 9.
Gene details: NCBI Gene ID: 9210; HGNC ID: HGNC:1068; UniProt: O95972; RefSeq (MANE Select): NM_005448.2; Chromosome location: Xp11.22
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.