SYBR Green qPCR primers for detecting human SF1 (splicing factor 1, also called BBP) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human SF1 qPCR Primer Set – splicing factor 1
Also known as: BBP, D11S636, MBBP, ZCCHC25, ZFM1, ZNF162
About this gene: SF1 encodes a nuclear pre-mRNA splicing factor. The SF1 protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor, and is required for the early stages of spliceosome assembly. The SF1 gene product also is involved in nuclear pre-mRNA retention and transcriptional repression.
Gene details: NCBI Gene ID: 7536; HGNC ID: HGNC:12950; UniProt: Q15637; RefSeq (MANE Select): NM_004630.4; Chromosome location: 11q13.1
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.