SYBR Green qPCR primers for detecting human SEMA5A (semaphorin 5A, also called Semaphorin-F) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human SEMA5A qPCR Primer Set – semaphorin 5A
Also known as: SEMAF, semF
About this gene: SEMA5A belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and multiple thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. SEMA5A has been implicated as an autism susceptibility gene.
Gene details: NCBI Gene ID: 9037; HGNC ID: HGNC:10736; UniProt: Q13591; RefSeq (MANE Select): NM_003966.3; Chromosome location: 5p15.31
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.