SYBR Green qPCR primers for detecting human BEST3 (bestrophin 3) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human BEST3 qPCR Primer Set – bestrophin 3
Also known as: VMD2L3
About this gene: BEST3 belongs to the bestrophin family of anion channels, which includes BEST1, the gene mutant in vitelliform macular dystrophy, and 2 other BEST1-like genes, BEST2 and BEST4. Bestrophins are transmembrane proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries.
Gene details: NCBI Gene ID: 144453; HGNC ID: HGNC:17105; UniProt: Q8N1M1; RefSeq (MANE Select): NM_032735.3; Chromosome location: 12q15
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.