SYBR Green qPCR primers for detecting human RPA2 (replication protein A2) gene expression by real-time PCR. Each set comes with primer sequences and reaction conditions.
Human RPA2 qPCR Primer Set – replication protein A2
Also known as: REPA2, RP-A p32, RP-A p34, RPA32
About this gene: RPA2 encodes a subunit of the heterotrimeric Replication Protein A complex, which binds to single-stranded DNA, forming a nucleoprotein complex that is important for DNA metabolism, being involved in DNA replication, repair, recombination, telomere maintenance. The RPA complex protects single-stranded DNA from nucleases, prevents formation of secondary structures that would interfere with repair, and co-ordinates the recruitment and departure of different genome maintenance factors. The heterotrimeric complex has two different modes of ssDNA binding, a low-affinity and high-affinity mode, determined by which oligonucleotide/oligosaccharide-binding domains of the complex are utilized, and differing in the length of DNA bound.
Gene details: NCBI Gene ID: 6118; HGNC ID: HGNC:10290; UniProt: P15927; RefSeq (MANE Select): NM_002946.5; Chromosome location: 1p35.3
What you get: Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with H2O as needed prior to use. This amount is sufficient for 1,000 PCR reactions of 20 µl, assuming a final primer concentration of 0.1 µM.
Browse all Human PCR Primer Sets.